Open AccessCryopreservation: Paper‐Based Cell Cryopreservation (Adv. Biosys. 3/2020)
Author(s) -
Alnemari Roaa,
Sukumar Pavithra,
Deliorman Muhammedin,
Qasaimeh Mohammad A.
Publication year - 2020
Publication title -
advanced biosystems
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.153
H-Index - 18
ISSN - 2366-7478
DOI - 10.1002/adbi.202070033
Subject(s) - cryopreservation , spheroid , microbiology and biotechnology , hela , confocal microscopy , biology , cell , cell culture , genetics , embryo
In article number 1900203, Mohammad A. Qasaimeh and co‐workers utilize filter paper as a unique platform for long‐term cryopreservation of mammalian cells. Paper fibers (blue) offer 3D‐structural support for cells during cryopreservation and maintain the growth of 3D cell culture and spheroid formations before and after cryopreservation. The confocal image shows a HeLa spheroid formed post‐cryopreservation and grown within the paper platform (red: F‐actin, blue: nuclei, green: tubulin).