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Review of Stimulated Raman Scattering Microscopy Techniques and Applications in the Biosciences
Author(s) -
Li Yanping,
Shen Binglin,
Li Shaowei,
Zhao Yihua,
Qu Junle,
Liu Liwei
Publication year - 2021
Publication title -
advanced biology
Language(s) - English
Resource type - Journals
ISSN - 2701-0198
DOI - 10.1002/adbi.202000184
Subject(s) - microscopy , raman scattering , raman spectroscopy , nanotechnology , raman microspectroscopy , materials science , optical microscope , optics , physics , scanning electron microscope , composite material
Abstract Stimulated Raman scattering (SRS) microscopy is a nonlinear optical imaging method for visualizing chemical content based on molecular vibrational bonds. Featuring high speed, high resolution, high sensitivity, high accuracy, and 3D sectioning, SRS microscopy has made tremendous progress toward biochemical information acquisition, cellular function investigation, and label‐free medical diagnosis in the biosciences. In this review, the principle of SRS, system design, and data analysis are introduced, and the current innovations of the SRS system are reviewed. In particular, combined with various bio‐orthogonal Raman tags, the applications of SRS microscopy in cell metabolism, tumor diagnosis, neuroscience, drug tracking, and microbial detection are briefly examined. The future prospects for SRS microscopy are also shared.