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Efficient Human Cell Coexpression System and Its Application to the Production of Multiple Coronavirus Antigens
Author(s) -
Kim Chonsaeng,
Jeong You Kyeong,
Yu Jihyeon,
Shin Hye Jin,
Ku Keun Bon,
Cha Hyung Jin,
Han Jun Hee,
Hong SungAh,
Kim BumTae,
Kim SeongJun,
Woo JaeSung,
Bae Sangsu
Publication year - 2021
Publication title -
advanced biology
Language(s) - English
Resource type - Journals
ISSN - 2701-0198
DOI - 10.1002/adbi.202000154
Subject(s) - biology , antigen , gene , coronavirus , computational biology , genome , human genome , coronaviridae , cell culture , virology , genetics , covid-19 , infectious disease (medical specialty) , disease , medicine , pathology
Coproduction of multiple proteins at high levels in a single human cell line would be extremely useful for basic research and medical applications. Here, a novel strategy for the stable expression of multiple proteins by integrating the genes into defined transcriptional hotspots in the human genome is presented. As a proof‐of‐concept, it is shown that EYFP is expressed at similar levels from hotspots and that the EYFP expression increases proportionally with the copy number. It is confirmed that three different fluorescent proteins, encoded by genes integrated at different loci, can be coexpressed at high levels. Further, a stable cell line is generated, producing antigens from different human coronaviruses: MERS‐CoV and HCoV‐OC43. Antibodies raised against these antigens, which contain human N‐glycosylation, show neutralizing activities against both viruses, suggesting that the coexpression system provides a quick and predictable way to produce multiple coronavirus antigens, such as the recent 2019 novel human coronavirus.