Open AccessHydrogels: 3D Microenvironment Stiffness Regulates Tumor Spheroid Growth and Mechanics via p21 and ROCK (Adv. Biosys. 9/2019)
Author(s) -
Taubenberger Anna V.,
Girardo Salvatore,
Träber Nicole,
FischerFriedrich Elisabeth,
Kräter Martin,
Wagner Katrin,
Kurth Thomas,
Richter Isabel,
Haller Barbara,
Binner Marcus,
Hahn Dominik,
Freudenberg Uwe,
Werner Carsten,
Guck Jochen
Publication year - 2019
Publication title -
advanced biosystems
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.153
H-Index - 18
ISSN - 2366-7478
DOI - 10.1002/adbi.201970092
Subject(s) - spheroid , self healing hydrogels , confocal microscopy , biophysics , confocal , stiffness , chemistry , actin , tumor microenvironment , materials science , biomedical engineering , microbiology and biotechnology , optics , biology , composite material , tumor cells , physics , cancer research , polymer chemistry , medicine , biochemistry , in vitro
In article number 1900128, Anna V. Taubenberger and co‐workers employ fluorescently labelled polyacrylamide beads (Cy3, green) as stress sensors that are incorporated into hydrogels together with breast cancer cells. After 14 days, tumor spheroid cultures were fixed, stained for F‐actin (red) and nuclei (blue), and imaged via confocal microscopy (maximum projection shown). In stiff hydrogels, the beads are deformed in proximity to the spheroid surface, which indicates that the spheroids experience radial compressive stress.