Open AccessBionanotechnology: Peptide‐Mediated Liposome Fusion as a Tool for the Detection of Matrix Metalloproteinases (Adv. Biosys. 5/2019)
Author(s) -
Mazur Federico,
Chandrawati Rona
Publication year - 2019
Publication title -
advanced biosystems
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.153
H-Index - 18
ISSN - 2366-7478
DOI - 10.1002/adbi.201970053
Subject(s) - liposome , matrix metalloproteinase , peptide , fluorophore , fusion , chemistry , matrix (chemical analysis) , biophysics , biochemistry , fluorescence , biology , physics , chromatography , optics , linguistics , philosophy
In article number 1800330 , Rona Chandrawati and Federico Mazur report the fusion of fluorophore‐containing liposomes mediated by complementary coiled‐coil‐forming peptides, which forms the basis of a detection assay for matrix metalloproteinases (MMPs). Fusion events are detected by an emission of light upon excitation of the donor fluorophores. In the presence of MMPs, the enzyme specifically cleaves the peptide on liposome surfaces, inhibiting peptide‐mediated liposome fusion.