Open AccessEnzyme Immobilization: Overcoming the Pitfalls of Cytochrome P450 Immobilization through the Use of Fusogenic Liposomes (Adv. Biosys. 1/2019)
Author(s) -
Kiiski Iiro M. A.,
Pihlaja Tea,
Urvas Lauri,
Witos Joanna,
Wiedmer Susanne K.,
Jokinen Ville P.,
Sikanen Tiina M.
Publication year - 2019
Publication title -
advanced biosystems
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.153
H-Index - 18
ISSN - 2366-7478
DOI - 10.1002/adbi.201970012
Subject(s) - liposome , biotinylation , enzyme , chemistry , immobilized enzyme , lipid bilayer , cytochrome p450 , membrane , drug delivery , biophysics , cytochrome , biochemistry , biology , organic chemistry
Immobilization of drug metabolizing enzymes on solid supports improves prediction of drug‐drug interactions by enabling enzyme inhibition assays under flow conditions. In article number 1800245 , Tiina M. Sikanen and co‐workers use fusogenic liposomes for biotinylation of lipid bilayers to facilitate immobilization of the membrane‐bound cytochrome P450s on avidin‐functionalized microfluidic devices. Preservation of the full complexity of the lipid environment ensures that the authentic enzyme kinetic parameters can be maintained.