Open AccessProteomic and Metabolomic Characterization of Human Neurovascular Unit Cells in Response to Methamphetamine
Author(s) -
Herland Anna,
Maoz Ben M.,
FitzGerald Edward A.,
Grevesse Thomas,
Vidoudez Charles,
Sheehy Sean P.,
Budnik Nikita,
Dauth Stephanie,
Mannix Robert,
Budnik Bogdan,
Parker Kevin Kit,
Ingber Donald E.
Publication year - 2020
Publication title -
advanced biosystems
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.153
H-Index - 18
ISSN - 2366-7478
DOI - 10.1002/adbi.201900230
Subject(s) - metabolome , methamphetamine , astrocyte , blood–brain barrier , pericyte , metabolomics , cell type , human brain , cell , central nervous system , biology , neuroscience , proteomics , microbiology and biotechnology , chemistry , pharmacology , in vitro , endothelial stem cell , bioinformatics , biochemistry , gene
The functional state of the neurovascular unit (NVU), composed of the blood–brain barrier and the perivasculature that forms a dynamic interface between the blood and the central nervous system (CNS), plays a central role in the control of brain homeostasis and is strongly affected by CNS drugs. Human primary brain microvascular endothelium, astrocyte, pericyte, and neural cell cultures are often used to study NVU barrier functions as well as drug transport and efficacy; however, the proteomic and metabolomic responses of these different cell types are not well characterized. Culturing each cell type separately, using deep coverage proteomic analysis and characterization of the secreted metabolome, as well as measurements of mitochondrial activity, the responses of these cells under baseline conditions and when exposed to the NVU‐impairing stimulant methamphetamine (Meth) are analyzed. These studies define the previously unknown metabolic and proteomic profiles of human brain pericytes and lead to improved characterization of the phenotype of each of the NVU cell types as well as cell‐specific metabolic and proteomic responses to Meth.