Open AccessCas9 Ribonucleoprotein Delivery via Microfluidic Cell‐Deformation Chip for Human T‐Cell Genome Editing and Immunotherapy
Author(s) -
Han Xin,
Liu Zongbin,
Ma Yuan,
Zhang Kai,
Qin Lidong
Publication year - 2017
Publication title -
advanced biosystems
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.153
H-Index - 18
ISSN - 2366-7478
DOI - 10.1002/adbi.201600007
Subject(s) - genome editing , ribonucleoprotein , cell , immunotherapy , cas9 , microfluidics , lab on a chip , crispr , microfluidic chip , genome , microbiology and biotechnology , chemistry , computational biology , nanotechnology , biology , materials science , immunology , rna , gene , biochemistry , immune system
This study reports a microfluidic cell deformation‐based method to deliver the Cas9 ribonucleoprotein (RNP) complexes to different cell types for efficient genome editing, including hard‐to‐transfect human primary CD4+ T cells. The RNP based CRISPR‐Cas9 system has great advantage in shortening reaction time and reducing off‐target problems, which holds great potential in future gene therapy applications.