Cas9 Ribonucleoprotein Delivery via Microfluidic Cell‐Deformation Chip for Human T‐Cell Genome Editing and Immunotherapy | Zendy

Han Xin | Zendy; Liu Zongbin | Zendy; Ma Yuan | Zendy; Zhang Kai | Zendy; Qin Lidong | Zendy

Open Access

Cas9 Ribonucleoprotein Delivery via Microfluidic Cell‐Deformation Chip for Human T‐Cell Genome Editing and Immunotherapy

Author(s) -

Han Xin,

Liu Zongbin,

Ma Yuan,

Zhang Kai,

Qin Lidong

Publication year - 2017

Publication title -

advanced biosystems

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.153

H-Index - 18

ISSN - 2366-7478

DOI - 10.1002/adbi.201600007

Subject(s) - ribonucleoprotein , genome editing , cas9 , crispr , transfection , cell , microfluidics , computational biology , genome , microbiology and biotechnology , biology , gene , nanotechnology , genetics , materials science , rna

This study reports a microfluidic cell deformation‐based method to deliver the Cas9 ribonucleoprotein (RNP) complexes to different cell types for efficient genome editing, including hard‐to‐transfect human primary CD4+ T cells. The RNP based CRISPR‐Cas9 system has great advantage in shortening reaction time and reducing off‐target problems, which holds great potential in future gene therapy applications.

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