Open AccessMixed‐solid substrate fermentation. A novel process for enhanced lipase production by Candida rugosa
Author(s) -
Benjamin S.,
Pandey A.
Publication year - 1998
Publication title -
acta biotechnologica
Language(s) - English
Resource type - Journals
eISSN - 1521-3846
pISSN - 0138-4988
DOI - 10.1002/abio.370180405
Subject(s) - food science , fermentation , chemistry , maltose , candida rugosa , substrate (aquarium) , lipase , yeast extract , yield (engineering) , hydrolysis , corn steep liquor , bran , biochemistry , biology , raw material , sucrose , enzyme , organic chemistry , materials science , ecology , metallurgy
Candida rugosa was cultivated in a mixed‐solid substrate containing coconut oil cake (COC) and fine and coarse wheat bran (1:1:1) with an initial water activity (a w ) of 0.92. The substrate was modified by adding a mineral solution (5%), corn steep liquor (6%), maltose (2%), peptone (3%), olive oil (10%), gum arabic (0.4%), different fatty acids (0.3%) and Tweens (0.5%). Fermentation in a column fermenter significantly improved the lipase yield to 118.2 Units per gram of dry fermented substrate [U/gds] at 72 h. This result was obtained 24 hours earlier than in our former studies (87.76 U/gds at 96 h) in COC, and the yield showed a 38% increase. Growth was measured indirectly by determining the glucosamine content in the cell wall of the yeast contained in the fermented matter, after its hydrolysis.