Open AccessGrowth and Quantification of MERS‐CoV Infection
Author(s) -
Coleman Christopher M.,
Frieman Matthew B.
Publication year - 2015
Publication title -
current protocols in microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 35
eISSN - 1934-8533
pISSN - 1934-8525
DOI - 10.1002/9780471729259.mc15e02s37
Subject(s) - middle east respiratory syndrome coronavirus , biology , vero cell , virology , middle east respiratory syndrome , virus , coronavirus , viral replication , titer , covid-19 , infectious disease (medical specialty) , disease , medicine , pathology
Middle East respiratory syndrome coronavirus (MERS‐CoV) is an emerging highly pathogenic respiratory virus. Although MERS‐CoV only emerged in 2012, we and others have developed assays to grow and quantify infectious MERS‐CoV and RNA products of replication in vitro. MERS‐CoV is able to infect a range of cell types, but replicates to high titers in Vero E6 cells. Protocols for the propagation and quantification of MERS‐CoV are presented. © 2015 by John Wiley & Sons, Inc.