Open AccessExpansion of Human Embryonic Stem Cells In Vitro
Author(s) -
Costa Magdaline,
Sourris Koula,
Hatzistavrou Tanya,
Elefanty Andrew G.,
Stanley Edouard G.
Publication year - 2008
Publication title -
current protocols in stem cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.658
H-Index - 28
eISSN - 1938-8969
pISSN - 1941-7322
DOI - 10.1002/9780470151808.sc01c01s5
Subject(s) - biology , embryonic stem cell , stem cell , microbiology and biotechnology , in vitro , genetics , gene
This unit describes a protocol for the large‐scale expansion of karyotypically normal human embryonic stem cells (hESCs). hESCs can be maintained indefinitely as dense colonies that are mechanically cut into pieces, which are subsequently transferred to fresh organ culture dishes seeded with primary mouse embryonic fibroblasts (MEFs). hESCs can also be enzymatically passaged (bulk culture); however, over time, this style of culturing may lead to the acquisition of chromosomal abnormalities. Nevertheless, enzymatic passaging can be used for short periods (up to 25 passages) without the appearance of cells with abnormal karyotypes. Curr. Protoc. Stem Cell Biol . 5:1C.1.1‐1C.1.7. © 2008 by John Wiley & Sons, Inc.