Open AccessHuman ABCE1 exhibits temperature‐dependent heterologous co‐functionality in S. cerevisiae
Author(s) -
Wada Miki,
Ito Koichi
Publication year - 2022
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.13463
Subject(s) - heterologous , saccharomyces cerevisiae , complementation , heterologous expression , chemistry , yeast , gene knockout , biology , microbiology and biotechnology , gene , biochemistry , recombinant dna , phenotype
ABCE1 protein (Rli1 in Saccharomyces cerevisiae ) is a unique ribosome recycling factor that is composed of an N‐terminal FeS cluster domain and two ATPase domains. Here, we report that heterologous expression of human ABCE1 in S. cerevisiae is unable to complement conditional knockout of ABCE1 (Rli1), at a typical experimental temperature of 30 °C. However, low but significant growth was observed at high temperature, 37 °C. Considering the close interaction of ABCE1 with translation factors and ribosomal components, the observed temperature‐dependent complementation may be attributed to heterologous co‐functionality of ABCE1 with S. cerevisiae factor(s), and might reflect functional upregulation of human ABCE1 at its functional temperature.