Open AccessHistidine is essential for growth of Komagataella phaffii cultured in YPA medium
Author(s) -
Gupta Aditi,
Rangarajan Pundi N.
Publication year - 2022
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.13408
Subject(s) - histidine , auxotrophy , pichia pastoris , biochemistry , yeast , biology , chemically defined medium , enzyme , chemistry , gene , recombinant dna , escherichia coli , in vitro
Komagataella phaffii ( a.k.a. Pichia pastoris ) requires histidine for optimal growth when cultured in a medium containing yeast extract, peptone (YP), and acetate (YPA). We demonstrate that HIS4 ‐deficient, K. phaffii strain GS115 exhibits a growth defect on YP‐media containing acetate, but not on other carbon sources. K. phaffii X33 , a prototroph, grows better than K. phaffii GS115 ( his4 ), a histidine auxotroph in YPA. Normal growth of GS115 is restored either by the expression of HIS4 or by culturing in YPA containing ≥0.6 mM histidine. In the presence of histidine, expression of several genes is altered, including those encoding key subunits of mitochondrial ATP synthase, transporters of amino acids and nutrients, as well as biosynthetic enzymes. Thus, histidine should be included as an essential component for optimal growth of K . phaffii histidine auxotrophs cultured in YPA.