Open AccessImprovement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging
Author(s) -
Hennig Simon,
Manstein Dietmar J.
Publication year - 2021
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.13246
Subject(s) - confocal , microscope , resolution (logic) , confocal microscopy , microscopy , optics , quantum dot , image resolution , confocal laser scanning microscopy , diffraction , materials science , superresolution , laser , scanning confocal electron microscopy , nanotechnology , physics , computer science , computer vision , biophysics , image (mathematics) , artificial intelligence , biology
Super‐resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy‐to‐use sub‐diffraction imaging method that achieves an almost 2‐fold improvement in resolution when used with conventional confocal microscopes. Here, we report an overall 3‐fold increase in lateral and axial resolution compared to conventional confocal microscopes by combining QDTI with state‐of‐the‐art commercial laser scanning microscope systems.