Open AccessRole of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout
Author(s) -
Ye Xiaogang,
Zhao Shuo,
Wu Meiyu,
Ruan Jinghua,
Tang Xiaoli,
Wang Xiaoxiao,
Zhong Boxiong
Publication year - 2021
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.13239
Subject(s) - sericin , mutant , silk , transcriptome , gene , biology , immune system , secretion , wild type , microbiology and biotechnology , gene knockout , gene expression , biochemistry , genetics , computer science , operating system
The domestic silkworm is a type of lepidopteran insect that feeds on mulberry leaves and has high economic value because of its ability to spin cocoons. Sericin 1 is an important component of silkworm cocoons, accounting for approximately 25% of the material. In this study, CRISPR/Cas9‐mediated gene editing was successfully used to destroy the sericin 1 gene, and homozygous mutants were obtained after continuous screening. Homozygous mutation resulted in premature termination of the translation of sericin 1 protein at 323 amino acids. Comparative transcriptomic and proteomic analyses of middle silk gland cells from wild‐type individuals and mutants were performed on the fourth day of the fifth instar, and the results suggest that sericin 1 plays an important role in the cellular immune system. In addition, the results suggest that sericin 1 has a synergistic effect with some protease inhibitors and that the secretion of these proteins is strictly regulated. These results will provide new insights into the function and expression pattern of sericin 1 and the mechanism of silk secretion.