Open AccessWheat‐ghretropins: novel ghrelin‐releasing peptides derived from wheat protein
Author(s) -
Tanikawa Kana,
Kaneko Kentaro,
Abe Shimon,
Nakato Jyunya,
Tokuyama Yuki,
Odaka Sayano,
Iwakura Hiroshi,
Sato Masaru,
Kurabayashi Atsushi,
Suzuki Hideyuki,
Makita Miki,
Ikemoto Hiroyuki,
Matsumura Shigenobu,
Inoue Kazuo,
Ohinata Kousaku
Publication year - 2021
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.13124
Subject(s) - ghrelin , orexigenic , peptide , secretion , appetite , chemistry , endocrinology , medicine , peptide hormone , in vivo , hormone , biochemistry , biology , receptor , neuropeptide , microbiology and biotechnology , neuropeptide y receptor
Ghrelin is an endogenous orexigenic hormone mainly produced by stomach cells and is reported to influence appetite, gastrointestinal motility and growth hormone secretion. We observed that enzymatic digest of wheat gluten stimulated ghrelin secretion from mouse ghrelinoma 3‐1, a ghrelin‐releasing cell line. Further on, we characterized the ghrelin‐releasing peptides present in the digest by comprehensive peptide analysis using liquid chromatography–mass spectrometry and structure–activity relationship. Among the candidate peptides, we found that SQQQQPVLPQQPSF, LSVTSPQQVSY and YPTSL stimulated ghrelin release. We then named them wheat‐ghretropin A, B and C, respectively. In addition, we observed that wheat‐ghretropin A increased plasma ghrelin concentration and food intake in mice after oral administration. Thus, we demonstrated that wheat‐ghretropin stimulates ghrelin release both in vitro and in vivo . To the best of our knowledge, this is the first report of a wheat‐derived exogenous bioactive peptide that stimulates ghrelin secretion.