Open AccessEnvironmental pH stress influences cellular secretion and uptake of extracellular vesicles
Author(s) -
Nakase Ikuhiko,
Ueno Natsumi,
Matsuzawa Mie,
Noguchi Kosuke,
Hirano Mami,
Omura Mika,
Takenaka Tomoya,
Sugiyama Ayaka,
Bailey Kobayashi Nahoko,
Hashimoto Takuya,
TakataniNakase Tomoka,
Yuba Eiji,
Fujii Ikuo,
Futaki Shiroh,
Yoshida Tetsuhiko
Publication year - 2021
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.13107
Subject(s) - microvesicles , tetraspanin , secretion , intracellular , extracellular , hela , microbiology and biotechnology , cd63 , cell , cell culture , microvesicle , extracellular vesicle , chemistry , vesicle , biology , biochemistry , microrna , membrane , gene , genetics
Exosomes (extracellular vesicles/EVs) participate in cell–cell communication and contain bioactive molecules, such as microRNAs. However, the detailed characteristics of secreted EVs produced by cells grown under low pH conditions are still unknown. Here, we report that low pH in the cell culture medium significantly affected the secretion of EVs with increased protein content and zeta potential. The intracellular expression level and location of stably expressed GFP‐fused CD63 (an EV tetraspanin) in HeLa cells were also significantly affected by environmental pH. In addition, increased cellular uptake of EVs was observed. Moreover, the uptake rate was influenced by the presence of serum in the cell culture medium. Our findings contribute to our understanding of the effect of environmental conditions on EV‐based cell–cell communication.