Open AccessComparison of separation methods for tissue‐derived extracellular vesicles in the liver, heart, and skeletal muscle
Author(s) -
Matejovič Adam,
Wakao Shohei,
Kitada Masaaki,
Kushida Yoshihiro,
Dezawa Mari
Publication year - 2021
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.13075
Subject(s) - extracellular vesicles , skeletal muscle , vesicle , intracellular , organelle , microbiology and biotechnology , ultracentrifuge , extracellular , chemistry , exosome , in vitro , biochemistry , microvesicles , biology , anatomy , membrane , microrna , gene
Extracellular vesicles (EVs), which are nanosized vesicles released by cells as intracellular messengers, have high potential as biomarkers. EVs are usually collected from in vitro sources, such as cell culture media or biofluids, and not from tissues. Techniques enabling direct collection of EVs from tissues will extend the applications of EVs. We compared methods for separating EVs from solid liver, heart, and skeletal muscle. Compared with a precipitation method, an ultracentrifugation‐based method for collection of EVs from solid tissues yielded a higher proportion of EVs positive for EV‐related markers, with minimum levels of intracellular organelle‐related markers. Some tissue‐specific modifications, such as a sucrose cushion step, may improve the yield and purity of the collected EVs.