Open AccessGenetic deletion of the 67‐kDa isoform of glutamate decarboxylase alters conditioned fear behavior in rats
Author(s) -
Fujihara Kazuyuki,
Sato Takumi,
Miyasaka Yoshiki,
Mashimo Tomoji,
Yanagawa Yuchio
Publication year - 2021
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.13065
Subject(s) - glutamate decarboxylase , fear conditioning , knockout mouse , gabaergic , gene knockout , extinction (optical mineralogy) , conditional gene knockout , neuroscience , cued speech , psychology , cognition , endocrinology , biology , medicine , gene , genetics , amygdala , enzyme , biochemistry , receptor , paleontology , phenotype , inhibitory postsynaptic potential , cognitive psychology
Inhibitory neurotransmitter GABA is synthesized by two enzymes, the glutamate decarboxylase 67‐kDa isoform (GAD67) and the 65‐kDa isoform (GAD65). Knocking out the Gad1 gene, which encodes GAD67, is lethal in mice, and therefore, the association between Gad1 and emotional function was unclear. In the present study, we assessed the impact of Gad1 knockout on fear expression and fear memory for the first time, using newly generated knockout rats.