Open AccessPLCγ1 inhibition combined with inhibition of apoptosis and necroptosis increases cartilage matrix synthesis in IL‐1β‐treated rat chondrocytes
Author(s) -
Chen Xiaolei,
Chen Ri,
Xu Yang,
Xia Chun
Publication year - 2021
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.13064
Subject(s) - necroptosis , chondrocyte , aggrecan , apoptosis , cartilage , programmed cell death , microbiology and biotechnology , osteoarthritis , medicine , matrix metalloproteinase , type ii collagen , cancer research , pharmacology , immunology , chemistry , biology , pathology , biochemistry , anatomy , alternative medicine , articular cartilage
We found that inhibition of PLCγ1 phosphorylation by U73122 improved cartilage matrix synthesis in IL‐1β‐treated rat chondrocytes. However, it also increased cell apoptosis and necroptosis, which reduced the effect on cartilage matrix synthesis. Inhibition of programmed cell death by simultaneous treatment with apoptosis and necroptosis inhibitors increased chondrocyte proliferation and further enhanced cartilage matrix synthesis.