Open AccessInactivation of the three GGA genes in HeLa cells partially compromises lysosomal enzyme sorting
Author(s) -
Doray Balraj,
Liu Lin,
Lee WangSik,
Jennings Benjamin C.,
Kornfeld Stuart
Publication year - 2021
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.13040
Subject(s) - golgi apparatus , cathepsin d , microbiology and biotechnology , mannose , cathepsin , hela , chemistry , biochemistry , lysosome , signal transducing adaptor protein , cathepsin b , receptor , biology , enzyme , cell
In this study, we inactivated all three GGA genes in HeLa cells using CRISPR‐Cas9 genome editing. The GGA triple‐knockout cells secreted 27% of newly synthesized cathepsin D, compared to 2.6% secretion seen with wild‐type HeLa cells. In contrast, HeLa cells with complete inactivation of the mannose 6‐phosphate pathway secreted 77.5% of the newly synthesized cathepsin D.