Inactivation of the three  GGA  genes in HeLa cells partially compromises lysosomal enzyme sorting | Zendy

Doray Balraj | Zendy; Liu Lin | Zendy; Lee WangSik | Zendy; Jennings Benjamin C. | Zendy; Kornfeld Stuart | Zendy

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Inactivation of the three GGA genes in HeLa cells partially compromises lysosomal enzyme sorting

Author(s) -

Doray Balraj,

Liu Lin,

Lee WangSik,

Jennings Benjamin C.,

Kornfeld Stuart

Publication year - 2021

Publication title -

febs open bio

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.718

H-Index - 31

ISSN - 2211-5463

DOI - 10.1002/2211-5463.13040

Subject(s) - golgi apparatus , cathepsin d , microbiology and biotechnology , mannose , cathepsin , hela , chemistry , biochemistry , lysosome , signal transducing adaptor protein , cathepsin b , receptor , biology , enzyme , cell

In this study, we inactivated all three GGA genes in HeLa cells using CRISPR‐Cas9 genome editing. The GGA triple‐knockout cells secreted 27% of newly synthesized cathepsin D, compared to 2.6% secretion seen with wild‐type HeLa cells. In contrast, HeLa cells with complete inactivation of the mannose 6‐phosphate pathway secreted 77.5% of the newly synthesized cathepsin D.

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