Open AccessIsolation and thermal stabilization of mouse ferroportin
Author(s) -
Deshpande Chandrika N.,
Azucenas Corbin R.,
Qiao Bo,
Nomura Norimichi,
Xin Vicky,
Font Josep,
Iwata So,
Ganz Tomas,
Nemeth Elizabeta,
Mackenzie Bryan,
Jormakka Mika
Publication year - 2021
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.13039
Subject(s) - ferroportin , hepcidin , transporter , microbiology and biotechnology , chemistry , efflux , transport protein , biochemistry , biophysics , biology , computational biology , gene , inflammation , immunology
Here, Deshpande et al . describe the production and purification of mouse ferroportin (Fpn), the only known mammalian iron efflux protein, and selective loop deletion constructs with enhanced thermal stability. Transport activity and hepcidin responsiveness of the constructs were assessed in Xenopus oocytes. The present work establishes a new protocol for the generation of stable Fpn protein in solution, constituting a platform for continued biophysical studies.