Open AccessThe nucleotide specificity of succinyl‐CoA synthetase of Plasmodium falciparum is not determined by charged gatekeeper residues alone
Author(s) -
Vashisht Kapil,
Singh Pallavi,
Verma Sonia,
Dixit Rajnikant,
Mishra Neelima,
Pandey Kailash C.
Publication year - 2021
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.13034
Subject(s) - plasmodium falciparum , enzyme , recombinant dna , biochemistry , protein subunit , nucleotide , substrate (aquarium) , chemistry , biology , stereochemistry , gene , ecology , malaria , immunology
Electrostatic interactions between charged gatekeeper residues of the enzyme and approaching substrates can effectively discriminate between similar substrates. We investigated whether the charged gatekeeper residues play any role in the substrate specificity of P. falciparum succinyl‐CoA synthetase ( Pf SCS). Our results demonstrated that charged gatekeeper residues in Pf SCS did alter the ATP binding but could not alter its substrate specificity, pointing toward other factors affecting the substate specificity of Pf SCS.