Open AccessCharacterization of a thermophilic cytochrome P450 of the CYP203A subfamily from Binh Chau hot spring in Vietnam
Author(s) -
Nguyen KimThoa,
Nguyen NgocLan,
Milhim Mohammed,
Nguyen VanTung,
Lai ThiHongNhung,
Nguyen HuyHoang,
Le ThiThanhXuan,
Phan ThiTuyetMinh,
Bernhardt Rita
Publication year - 2021
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.13033
Subject(s) - subfamily , hot spring , thermophile , spring (device) , cytochrome p450 , zoology , cytochrome b , biology , biochemistry , enzyme , gene , phylogenetic tree , paleontology , mechanical engineering , engineering
A predicted alkali‐thermophilic cytochrome P450‐T2 from the DNA database of Binh Chau hot spring was synthesized, expressed and purified. The properties of the enzyme, including the optimum pH and temperature, melting temperature and half‐life at 50 °C were characterized. P450‐T2 accepts electrons from the BmCPR‐Fdx2 system. l ‐Mimosine and emodin may be its putative substrates.