Open AccessThe CAG promoter maintains high‐level transgene expression in HEK293 cells
Author(s) -
Dou Yuanyuan,
Lin Yan,
Wang Tianyun,
Wang XiaoYin,
Jia Yanlong,
Zhao Chunpeng
Publication year - 2021
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.13029
Subject(s) - transgene , hek 293 cells , biology , microbiology and biotechnology , genetics , gene
Through flow cytometry and qPCR technology, the promoter vector for high expression of eGFP protein in HEK293F cells was optimized and verified with hepatitis B surface antigen. The CAG promoter vector can not only effectively increase and maintain the expression level of recombinant proteins, but also increase the expression of transgenes under low temperature conditions.