Open AccessIbpB‐bound substrate release in living cells as revealed by unnatural amino acid‐mediated photo‐crosslinking
Author(s) -
Shi Xiaodong,
Ezemaduka Anastasia N.
Publication year - 2020
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12957
Subject(s) - in vivo , substrate (aquarium) , chemistry , endogeny , biophysics , amino acid , biochemistry , biology , genetics , ecology
Small heat shock proteins (sHSPs) are known to bind partially folded proteins under stress conditions. In this study, by using photo‐crosslinking, we showed that the substrate binding of sHSP IbpB in living cells is reversible upon short‐time exposure at elevated temperature, providing in vivo evidence on the existence of substrate protein release at nonstress conditions.