A systematic mutational analysis identifies a 5‐residue proline tag that enhances the  in vivo  immunogenicity of a non‐immunogenic model protein | Zendy

Rahman Nafsoon | Zendy; Islam Mohammad Monirul | Zendy; Kibria Md. Golam | Zendy; Unzai Satoru | Zendy; Kuroda Yutaka | Zendy

Open Access

A systematic mutational analysis identifies a 5‐residue proline tag that enhances the in vivo immunogenicity of a non‐immunogenic model protein

Author(s) -

Rahman Nafsoon,

Islam Mohammad Monirul,

Kibria Md. Golam,

Unzai Satoru,

Kuroda Yutaka

Publication year - 2020

Publication title -

febs open bio

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.718

H-Index - 31

ISSN - 2211-5463

DOI - 10.1002/2211-5463.12941

Subject(s) - immunogenicity , chemistry , arginine , proline , in vivo , epitope , trypsin , peptide , biochemistry , antibody , biology , microbiology and biotechnology , amino acid , enzyme , immunology

Solubility controlling peptide tags (SCP‐tags) constructed with 5‐proline (C5P) and 5‐arginine (C5R) residues did not alter the secondary structure content nor the tertiary structure nor the monomeric state of BPTI‐19A, but significantly increased its in vivo immunogenicity, by up to 240‐ and 73‐fold, respectively, suggesting the potential of non‐oligomerizing SCP‐tags for increasing the immunogenicity of a non‐immunogenic protein.

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