Open AccessA systematic mutational analysis identifies a 5‐residue proline tag that enhances the in vivo immunogenicity of a non‐immunogenic model protein
Author(s) -
Rahman Nafsoon,
Islam Mohammad Monirul,
Kibria Md. Golam,
Unzai Satoru,
Kuroda Yutaka
Publication year - 2020
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12941
Subject(s) - immunogenicity , chemistry , arginine , proline , in vivo , epitope , trypsin , peptide , biochemistry , antibody , biology , microbiology and biotechnology , amino acid , enzyme , immunology
Solubility controlling peptide tags (SCP‐tags) constructed with 5‐proline (C5P) and 5‐arginine (C5R) residues did not alter the secondary structure content nor the tertiary structure nor the monomeric state of BPTI‐19A, but significantly increased its in vivo immunogenicity, by up to 240‐ and 73‐fold, respectively, suggesting the potential of non‐oligomerizing SCP‐tags for increasing the immunogenicity of a non‐immunogenic protein.