Open AccessTracking leukemic T‐cell transcriptional dynamics in vivo with a blood‐based reporter assay
Author(s) -
Tamayo Alfred G.,
Shukor Syukri,
Burr Alexandra,
Erickson Patrick,
Parekkadan Biju
Publication year - 2020
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12940
Subject(s) - in vivo , luciferase , jurkat cells , microbiology and biotechnology , cell culture , biology , cancer cell , cell , transcription factor , cancer research , cancer , immunology , gene , transfection , genetics , t cell , immune system
An engineered, circulating cancer cell line secreting a luciferase reporter captures constitutive and circadian clock‐driven transcription dynamics. This was observed in vitro in a continuous flow cell culture system, and in vivo detecting circadian luciferase plasma activity. This technique is rapid, noninvasive and can aid in investigating relationships between cancer cell signaling and behavior, such as diet or sleep.