Open AccessA new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers
Author(s) -
Zhang Biao,
Wang HaiYang,
Wang DongXing,
Zeng Quan,
Fan Zeng,
Xi JiaFei,
Nan Xue,
He LiJuan,
Zhou JunNian,
Pei XueTao,
Yue Wen
Publication year - 2020
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12932
Subject(s) - cancer stem cell , hepatocellular carcinoma , cancer research , stem cell , liver cancer , stem cell marker , biology , cell culture , cell , in vitro , cancer , cluster of differentiation , microbiology and biotechnology , genetics
We enriched a specific subpopulation of liver cancer stem cells using multiple surface markers and then cultured the cells with modified chemically defined medium to maintain the cancer stem cell properties for 2 weeks in vitro . This study may provide an effective in vitro model for the study of the biological properties of liver cancer stem cells and their use in targeted drug screening.