A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers | Zendy

Zhang Biao | Zendy; Wang HaiYang | Zendy; Wang DongXing | Zendy; Zeng Quan | Zendy; Fan Zeng | Zendy; Xi JiaFei | Zendy; Nan Xue | Zendy; He LiJuan | Zendy; Zhou JunNian | Zendy; Pei XueTao | Zendy; Yue Wen | Zendy

Open Access

A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers

Author(s) -

Zhang Biao,

Wang HaiYang,

Wang DongXing,

Zeng Quan,

Fan Zeng,

Xi JiaFei,

Nan Xue,

He LiJuan,

Zhou JunNian,

Pei XueTao,

Yue Wen

Publication year - 2020

Publication title -

febs open bio

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.718

H-Index - 31

ISSN - 2211-5463

DOI - 10.1002/2211-5463.12932

Subject(s) - cancer stem cell , hepatocellular carcinoma , cancer research , stem cell , liver cancer , stem cell marker , biology , cell culture , cell , in vitro , cancer , cluster of differentiation , microbiology and biotechnology , genetics

We enriched a specific subpopulation of liver cancer stem cells using multiple surface markers and then cultured the cells with modified chemically defined medium to maintain the cancer stem cell properties for 2 weeks in vitro . This study may provide an effective in vitro model for the study of the biological properties of liver cancer stem cells and their use in targeted drug screening.

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