Open AccessAlzheimer's disease risk modifier genes do not affect tau aggregate uptake, seeding or maintenance in cell models
Author(s) -
Kolay Sourav,
Diamond Marc I.
Publication year - 2020
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12928
Subject(s) - biology , genome wide association study , gene , gene knockout , crispr , hek 293 cells , microbiology and biotechnology , genetics , single nucleotide polymorphism , genotype
Alzheimer's disease (AD) is a major cause of dementia. The molecular basis of disease progression is unknown. We hypothesized that the identified AD genome‐wide association study (GWAS) genes would regulate this process. We used CRISPR‐mediated knockout of these genes to test their effects in the cell model systems of tau propagation. GWAS gene knockouts had no effect in these models of tau pathology.