Open AccessNeutrophil activation by Escherichia coli isolates from human intestine: effects of bacterial hydroperoxidase activity and surface hydrophobicity
Author(s) -
Moshkovskaya Mariam,
Vakhrusheva Tatyana,
Rakitina Daria,
Baykova Julia,
Panasenko Oleg,
Basyreva Lilia,
Gusev Sergey,
Gusev Alexander,
Mikhalchik Elena,
Smolitalia,
Dobretsov Gennadiy,
Scherbakov Petr,
Parfenov Asfold,
Fadeeva Nina,
Pobeguts Olga,
Govorun Vadim
Publication year - 2020
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12796
Subject(s) - catalase , escherichia coli , peroxidase , reactive oxygen species , microbiology and biotechnology , neutrophil extracellular traps , extracellular , biology , dysbiosis , innate immune system , bacteria , chemistry , inflammation , gut flora , immunology , biochemistry , immune system , enzyme , genetics , gene
Successful colonization of the intestine requires that bacteria interact with the innate immune system and, in particular, neutrophils. Progression of inflammatory bowel diseases (IBD) is associated with alterations in gut microbiota, and dysbiosis in Crohn’s disease (CD) patients is often associated with an expansion of Escherichia coli . Here, we investigated the ability of such E. coli isolates to avoid neutrophil activation and to utilize reactive oxygen species. Neutrophil activation was detected in vitro in normal human blood via luminol chemiluminescence (CL) induced by reactive oxygen and halogen species generated by neutrophils. No significant difference in neutrophil activation in vitro was detected between isolates from inflamed (23 isolates) vs healthy intestines (5 isolates), with 10‐fold variation within both groups (2.9–61.2 mV). CL activity of isolates from the same patient differed by 1.5–5 times. Twenty‐four isolates from ileal aspirate, biopsy, and feces of seven patients with CD and one patient with no intestine inflammation were tested for extracellular peroxidase and catalase activity and cell surface hydrophobicity. Average values between patients varied from 26 ± 3 to 73 ± 18 µmol·g −1 of air dry weight for peroxidase activity, from 15 ± 2 to 189 ± 56 mmol·g −1 of air dry weight for catalase activity, and from 5 ± 3 to 105 ± 9 a.u. for the hydrophobic probe fluorescence. Extracellular peroxidase activity and hydrophobicity of bacterial cell surface correlated negatively with stimulated neutrophil CL. The ability of some isolates to avoid neutrophil activation and to utilize reactive oxygen species may provide a strategy to survive assault by the innate immune system.