Open AccessCircular RNA expression profiles in extracellular vesicles from the plasma of patients with pancreatic ductal adenocarcinoma
Author(s) -
Li Qingqing,
Geng Shasha,
Yuan Huixiao,
Li Yang,
Zhang Shuxian,
Pu Lin,
Ge Jianli,
Niu Xianping,
Li Yandong,
Jiang Hua
Publication year - 2019
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12741
Subject(s) - circular rna , nanoparticle tracking analysis , microvesicles , microrna , pancreatic ductal adenocarcinoma , extracellular vesicles , biology , transcriptome , computational biology , rna , pancreatic cancer , exosome , microbiology and biotechnology , bioinformatics , gene , gene expression , genetics , cancer
Tumor‐derived extracellular vesicles (EVs) and their contents are involved in the development of human malignancies. Circular RNAs (circRNAs), enriched in EVs, can regulate diverse cellular processes by acting as microRNA (miRNA) sponges or through other mechanisms. In the present study, we explored the potential roles of circRNAs in EVs in the development of pancreatic ductal adenocarcinoma (PDAC). First, plasma was obtained from patients with PDAC ( n = 8) and healthy volunteers ( n = 8), and EVs were isolated by the ultracentrifugation method. Nanoparticle tracking analysis and transmission electron microscopy confirmed the size and form of the isolated EVs. The circRNA expression profiles of EVs were investigated by high‐throughput whole transcriptome sequencing. We then further validated the accuracy of the circRNA sequencing data by quantitative real‐time PCR analysis using plasma samples and PC cell lines, and subsequently performed bioinformatics analysis to reveal the potential functional roles of the differentially expressed circRNAs and to construct a circRNA‐miRNA interaction network to predict the target miRNAs of these circRNAs. Our work provides novel targets for further studies concerning the pathogenesis of PDAC.