Open AccessmiR‐122‐5p modulates the radiosensitivity of cervical cancer cells by regulating cell division cycle 25A ( CDC25A )
Author(s) -
Ding FengNa,
Gao BaoHong,
Wu Xia,
Gong ChunWu,
Wang WeiQing,
Zhang ShuMao
Publication year - 2019
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12730
Subject(s) - cdc25a , radiosensitivity , cell cycle , cell division , division (mathematics) , microbiology and biotechnology , biology , cancer research , cancer cell , cancer , cell cycle checkpoint , cell , medicine , genetics , radiation therapy , mathematics , arithmetic
Cervical cancer is one of the most common gynecological malignancies globally, Unfortunately, radiotherapy and chemotherapy are not effective at treating some cases of this disease, and the 5‐year survival rate is only 40–50%. Cell division cycle 25A ( CDC25A ) has been shown to induce radioresistance in a variety of tumor cells, but the role of CDC25A in the radioresistance of cervical cancer has not been fully elucidated. Here, we report that CDC25A is highly expressed and miR‐122‐5p lowly expressed in cervical cancer tissues and cells. The TargetScan database was used to predict CDC25A as a target of miR‐122‐5p, and the interactions between miR‐122‐5p and CDC25A were further confirmed by western blot, real‐time PCR and dual‐luciferase reporter assay. Under X‐ray irradiation, up‐regulation of CDC25A can promote the radiation resistance of cervical cancer cells, whereas overexpression of miR‐122‐5p or knockdown of CDC25A inhibits the survival and induces apoptosis of cervical cancer colonies. In conclusion, our data suggest that miR‐122‐5p enhances the radiosensitivity of cervical cancer cells by targeting CDC25A .