Open AccessDegradation kinetics of α‐conotoxin TxID
Author(s) -
Xu Pan,
Xiong Yang,
Liu Yiqiao,
Yu Shurun,
Zhangsun Dongting,
Wu Yong,
Luo Sulan
Publication year - 2019
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12697
Subject(s) - chemistry , degradation (telecommunications) , nicotinic acetylcholine receptor , chromatography , hydrolysis , conotoxin , kinetics , ionic strength , acetylcholine receptor , biochemistry , peptide , organic chemistry , aqueous solution , receptor , telecommunications , physics , computer science , quantum mechanics
α‐Conotoxin (CTx) TxID is a potent α3β4 nicotinic acetylcholine receptor (nAChR) antagonist that has been suggested as a potential drug candidate to treat addiction and small cell lung cancer. The function and structure of TxID have been well‐studied, but analyses of its stability have not previously been reported. The purpose of this study was to analyze the stability and forced degradation of TxID under various conditions: acid, alkali, water hydrolysis, oxidation, light, thiols, temperature, ionic strength and buffer pH. Different degradation products were formed under various conditions, and the degradation patterns of TxID showed pseudo‐first‐order kinetics. TxID degraded slowest at pH 3 within a pH range of 2–8. The major degradation products were analyzed using liquid chromatography–tandem mass spectrometry and the activity of the main product with α3β4 nAChR was analyzed using electrophysiological methods. Our analysis of TxID stability may aid the selection of appropriate conditions for peptide production, packaging and storage.