Open AccessSulfated polysaccharides interact with fibroblast growth factors and protect from denaturation
Author(s) -
Sun Changye,
Liu Mengxin,
Sun Panwen,
Yang Mingming,
Yates Edwin A.,
Guo Zhikun,
Fernig David G.
Publication year - 2019
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12696
Subject(s) - fibroblast growth factor , heparin , chondroitin sulfate , heparan sulfate , sulfation , chemistry , polysaccharide , biochemistry , glycosaminoglycan , dextran , denaturation (fissile materials) , microbiology and biotechnology , carrageenan , cell growth , fibroblast , biology , in vitro , receptor , nuclear chemistry
Fibroblast growth factors ( FGF s) regulate embryonic development and homeostasis, including tissue and organ repair and specific aspects of metabolism. The basic FGF and acidic FGF , now known as FGF 2 and FGF 1, are widely used protein drugs for tissue repair. However, they are susceptible to denaturation at ambient temperatures and during long‐time storage, which will reduce their biological activity. The interaction of FGF s with the sulfated domains of heparan sulfate and heparin is essential for their cellular signaling and stability. Therefore, we analyzed the interactions of FGF 1 and FGF 2 with four sulfated polysaccharides: heparin, dextran sulfate ( DXS ), λ‐carrageenan, and chondroitin sulfate. The results of thermal stability and cell proliferation assays demonstrate that heparin, DXS , and λ‐carrageenan bound to both FGF s and protected them from denaturation. Our results suggest heparin, DXS , and λ‐carrageenan are potential formulation materials that bind and stabilize FGF s, and which may also potentiate their activity and control their delivery.