Open AccessRetracted: Lnc RNA THOR increases osteosarcoma cell stemness and migration by enhancing SOX 9 mRNA stability
Author(s) -
Wu Haojun,
He Yanxia,
Chen Hang,
Liu Yanzhi,
Wei Bo,
Chen Guanghua,
Lin Han,
Lin Hao
Publication year - 2019
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12620
Subject(s) - gene knockdown , downregulation and upregulation , rna binding protein , messenger rna , untranslated region , cancer research , chemistry , three prime untranslated region , cell migration , microbiology and biotechnology , cell , biology , apoptosis , biochemistry , gene
Although the long non‐coding RNA THOR has been reported to promote cancer stem cell expansion in liver cancer and gastric cancer, its effects on osteosarcoma ( OS ) cells remain unclear. Here, we investigated the roles of THOR in the stemness and migration of OS cells. We report that the level of THOR is remarkably upregulated in OS cell spheroids compared to that in OS adherent cells. THOR overexpression increased spheroid formation ability and aldehyde dehydrogenase 1 ( ALDH 1) activity in OS adherent cells, and the opposite effect was observed in spheroids with THOR knockdown. Additionally, the spheroids formed by OS adherent cells exhibited a stronger migration ability, which was attenuated by THOR knockdown, and THOR overexpression increased OS cell migration. Mechanistically, mRNA stability, luciferase reporter, and RNA – RNA in vitro interaction assays indicated that THOR can directly bind to the middle region of the SOX9 3′‐untranslated region ( UTR ), and enhances its mRNA stability, thereby increasing its expression. Notably, SOX 9 knockdown reduced the ability of THOR overexpression to promote the stemness of OS cells. These findings indicate that the lnc RNA THOR can promote the stemness and migration of OS cells by directly binding to the middle region of SOX9 3′ UTR , thereby enhancing SOX9 mRNA stability and increasing its expression; thus, we provide information that may be of use in identifying potential targets for OS treatment.