Open AccessRecombinant yeast VDAC 2: a comparison of electrophysiological features with the native form
Author(s) -
Magrì Andrea,
Karachitos Andonis,
Di Rosa Maria Carmela,
Reina Simona,
Conti Nibali Stefano,
Messina Angela,
Kmita Hanna,
De Pinto Vito
Publication year - 2019
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12574
Subject(s) - recombinant dna , heterologous expression , saccharomyces cerevisiae , yeast , ion channel , biochemistry , gene isoform , voltage dependent anion channel , chemistry , lipid bilayer , heterologous , microbiology and biotechnology , biology , biophysics , escherichia coli , membrane , gene , bacterial outer membrane , receptor
Voltage‐dependent anion channel isoform 2 of the yeast Saccharomyces cerevisiae ( yVDAC 2) was believed for many years to be devoid of channel activity. Recently, we isolated yVDAC 2 and showed that it exhibits channel‐forming activity in the planar lipid bilayer system when in its so‐called native form. Here, we describe an alternative strategy for yVDAC 2 isolation, through heterologous expression in bacteria and refolding in vitro . Recombinant yVDAC 2, like its native form, is able to form voltage‐dependent channels. However, some differences between native and recombinant yVDAC 2 emerged in terms of voltage dependence and ion selectivity, suggesting that, in this specific case, the recombinant protein might be depleted of post‐translational modification(s) that occur in eukaryotic cells.