Open AccessCoprs inactivation leads to a derepression of LINE1 transposons in spermatocytes
Author(s) -
Paul Conception,
Delpech Hélène,
Haouzi Delphine,
Hamamah Samir,
Sardet Claude,
Fabbrizio Eric
Publication year - 2019
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12562
Subject(s) - piwi interacting rna , derepression , retrotransposon , protein arginine methyltransferase 5 , psychological repression , biology , histone , epigenetics , spermatocyte , transposable element , genetics , rna , microbiology and biotechnology , rna interference , methyltransferase , genome , methylation , gene , gene expression , meiosis
Repression of retrotransposons is essential for genome integrity during germ cell development and is tightly controlled through epigenetic mechanisms. In primordial germ cells, protein arginine N ‐methyltransferase (Prmt5) is involved in retrotransposon repression by methylating Piwi proteins, which is part of the pi RNA pathway. Here, we show that in mice, genetic inactivation of coprs (which is highly expressed in testis and encodes a histone‐binding protein required for the targeting of Prmt5 activity) affects the maturation of spermatogonia to spermatids. Mass spectrometry analysis revealed the presence of Miwi in testis protein lysates immunoprecipitated with an anti‐Coprs antibody. The observed deregulation of Miwi and pachytene pre‐pi RNA s levels and the derepression of LINE 1 repetitive sequences observed in coprs ‐/‐ mice suggest that Coprs is implicated in genome surveillance mechanisms.