Open AccessNephronectin mediates p38 MAPK ‐induced cell viability via its integrin‐binding enhancer motif
Author(s) -
Toraskar Jimita,
Magnussen Synnøve N.,
Chawla Konika,
Svineng Gunbjørg,
Steigedal Tonje S.
Publication year - 2018
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12544
Subject(s) - microbiology and biotechnology , mapk/erk pathway , integrin , phosphorylation , protein kinase a , intracellular , viability assay , enhancer , signal transduction , p38 mitogen activated protein kinases , biology , cell , chemistry , biochemistry , transcription factor , gene
Nephronectin ( NPNT ) is an extracellular matrix ( ECM ) protein involved in kidney development. We recently reported intracellular NPNT as a potential prognostic marker in breast cancer and that NPNT promotes metastasis in an integrin‐dependent manner. Here, we used reverse‐phase protein array ( RPPA ) to analyze NPNT ‐triggered intracellular signaling in the 66cl4 mouse breast cancer cell line. The results showed that the integrin‐binding enhancer motif is important for the cellular effects upon NPNT interaction with its receptors, including phosphorylation of p38 mitogen‐activated protein kinase ( MAPK ). Furthermore, analysis using prediction tools suggests involvement of NPNT in promoting cell viability. In conclusion, our results indicate that NPNT , via its integrin‐binding motifs, promotes cell viability through phosphorylation of p38 MAPK .