Open AccessCritical role of tristetraprolin and AU‐rich element RNA‐binding protein 1 in the suppression of cancer cell growth by globular adiponectin
Author(s) -
Tilija Pun Nirmala,
Khakurel Amrita,
Shrestha Aastha,
Kim SangHyun,
Park PilHoon
Publication year - 2018
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12541
Subject(s) - tristetraprolin , adiponectin , gene knockdown , apoptosis , messenger rna , cancer research , chemistry , cancer cell , cancer , microbiology and biotechnology , biology , rna binding protein , medicine , endocrinology , gene , biochemistry , insulin , insulin resistance
Adiponectin exhibits potent antitumor activities. Herein, we examined the molecular mechanisms underlying suppression of tumor growth by globular adiponectin ( gA crp). We demonstrated that gA crp suppressed B‐cell lymphoma 2 (Bcl‐2) expression, an anti‐apoptotic gene, by inducing its mRNA destabilization, which was accompanied with a decrease in cell viability and increased caspase‐3 activity in hepatic cancer cells. In addition, gA crp increased expression of tristetraprolin ( TTP ) and AU‐rich element RNA‐binding protein 1 ( AUF 1), which are mRNA stability regulatory proteins. Moreover, gA crp‐induced suppression of Bcl‐2 expression was abrogated by knockdown of TTP or AUF 1. These data indicate that gA crp induces apoptosis of hepatic cancer cells by TTP ‐ and AUF 1‐mediated Bcl‐2 mRNA destabilization, and further suggest that TTP and AUF 1 are novel targets mediating the antitumor activity of adiponectin.