Open AccessREBELOTE , a regulator of floral determinacy in Arabidopsis thaliana , interacts with both nucleolar and nucleoplasmic proteins
Author(s) -
de Bossoreille Stève,
Morel Patrice,
Trehin Christophe,
Negrutiu Ioan
Publication year - 2018
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12504
Subject(s) - nucleoplasm , nucleolus , ribosome biogenesis , ribosomal protein , arabidopsis thaliana , biology , microbiology and biotechnology , regulator , genetics , gene , rna , cytoplasm , ribosome , mutant
The nucleoplasm and nucleolus are the two main territories of the nucleus. While specific functions are associated with each of these territories (such as mRNA synthesis in the nucleoplasm and ribosomal rRNA synthesis in the nucleolus), some proteins are known to be located in both. Here, we investigated the molecular function of REBELOTE ( RBL ), an Arabidopsis thaliana protein previously characterized as a regulator of floral meristem termination. We show that RBL displays a dual localization, in the nucleolus and nucleoplasm. Moreover, we used direct and global approaches to demonstrate that RBL interacts with nucleic acid‐binding proteins. It binds to the NOC proteins SWA 2, At NOC 2 and At NOC 3 in both the nucleolus and nucleoplasm, and also to OBE 1 and VFP 3/ ENAP 1. Taking into account the identities of these RBL interactors, we hypothesize that RBL acts both in ribosomal biogenesis and in the regulation of gene expression.