Open AccessAcidic mammalian chitinase gene is highly expressed in the special oxyntic glands of Manis javanica
Author(s) -
Ma JingE,
Li LinMiao,
Jiang HaiYing,
Zhang XiuJuan,
Li Juan,
Li GuanYu,
Chen JinPing
Publication year - 2018
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12461
Subject(s) - chitinase , gene , biology , microbiology and biotechnology , biochemistry
The Malayan pangolin ( Manis javanica ) is a mammal that feeds primarily on ants and termites, which contain the energy‐rich carbohydrate chitin. Chitin is digestible by endogenous enzymes of the typical mammalian gastrointestinal tract, especially the acidic mammalian chitinase ( AMC ase). The objective of this research was to determine whether AMC ase activity is expressed in the stomach of M. javanica . The stomach tissues were divided into three parts: the gastric sack, the oxyntic glands, and the pyloric musculature, which were assayed by conventional RT ‐ PCR , quantitative reverse transcriptase‐coupled PCR ( qPCR ) and western blot. Information regarding 3D structural models of AMC ase was also obtained. In conclusion, acidic mammalian chitinase is highly expressed in the oxyntic glands of the M. javanica species.