Efficient CRISPR ‐based genome editing using tandem guide RNA s and editable surrogate reporters

Cleavage efficiency plays a key role in clustered regularly interspaced short palindromic repeat ( CRISPR )‐based gene editing, particularly when the given guide RNA exhibits low cleavage activity. Here, we describe the packaging of tandem guide RNA s and single‐strand annealing‐based surrogate reporter cassettes into the CRISPR /CRISPR‐associated protein 9 vector, which increased gene‐editing efficiency by 4.94–6.31‐fold and simultaneously enriched the proportion of genetically modified cells. This strategy may substantially improve genome‐editing efficiency for demanding applications.