Open AccessHistone methylation regulates Hif‐1 signaling cascade in activation of hepatic stellate cells
Author(s) -
Hong Fei,
Wan Lu,
Liu Jie,
Huang Ke,
Xiao Zhenmeng,
Zhang Yingjing,
Shi Chunwei
Publication year - 2018
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12379
Subject(s) - hepatic stellate cell , microbiology and biotechnology , histone methylation , histone h3 , histone , h3k4me3 , autophagy , chemistry , extracellular matrix , biology , dna methylation , gene expression , biochemistry , promoter , endocrinology , apoptosis , gene
Liver fibrosis is characterized by deposition of excessive extracellular matrix ( ECM ). The major source of ECM is activated hepatic stellate cells ( HSC s). Previously, we reported that hypoxia‐inducible factor‐1 (Hif‐1) regulates activation of HSC s through autophagy. In current work, human HSC cell line LX ‐2 was used as cell model. It was determined that trimethylation of H3 histone on lysine 4 (H3K4me3) occurred in the Hif‐1 transcriptional complex. Inhibition of modifications of histone methylation suppressed Hif‐1 nuclear transport, autophagosome formation, and activation of LX ‐2 cells. These data suggest that histone methylation modification plays an important role in the Hif‐1 signaling cascade regulating HSC activation.