Open AccessMicro RNA profiling during directed differentiation of cortical interneurons from human‐induced pluripotent stem cells
Author(s) -
Tu Jiajie,
Cao Dandan,
Li Lu,
Cheung HoiHung,
Chan WaiYee
Publication year - 2018
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12377
Subject(s) - neurogenesis , induced pluripotent stem cell , interneuron , biology , embryonic stem cell , directed differentiation , neuroscience , rna , developmental biology , cellular differentiation , neural stem cell , stem cell , microbiology and biotechnology , inhibitory postsynaptic potential , genetics , gene
Induced pluripotent stem cells ( iPSC s) are useful for modeling neuron development and related diseases. Cortical interneurons are essential players in neuropsychiatric diseases such as autism. mi RNA s are a class of pivotal regulators in neural differentiation. Using a previously established model of cortical interneuron differentiation from human embryonic stem cells, we profiled mi RNA s involved in differentiation from human iPSC s. A number of mi RNA s were modulated in the differentiation process. This study captured the temporal in vitro neurogenesis from iPSC s to mature cortical interneurons. The specific mi RNA s identified at each stage of differentiation are of potential use for drug discovery and prospective clinical applications.