Open AccessThe Stl repressor from Staphylococcus aureus is an efficient inhibitor of the eukaryotic fruitfly dUTP ase
Author(s) -
Benedek András,
Pölöskei István,
Ozohanics Olivér,
Vékey Károly,
Vértessy Beáta G.
Publication year - 2018
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12302
Subject(s) - dna , drosophila melanogaster , dna repair , repressor , biology , chemistry , biochemistry , microbiology and biotechnology , gene , gene expression
DNA metabolism and repair is vital for the maintenance of genome integrity. Specific proteinaceous inhibitors of key factors in this process have high potential for deciphering pathways of DNA metabolism and repair. The dUTP ase enzyme family is responsible for guarding against erroneous uracil incorporation into DNA . Here, we investigate whether the staphylococcal Stl repressor may interact with not only bacterial but also eukaryotic dUTP ase. We provide experimental evidence for the formation of a strong complex between Stl and Drosophila melanogaster dUTP ase. We also find that dUTP ase activity is strongly diminished in this complex. Our results suggest that the dUTP ase protein sequences involved in binding to Stl are at least partially conserved through evolution from bacteria to eukaryotes.