Open AccessThe proteomic profile of a mouse model of proliferative vitreoretinopathy
Author(s) -
Márkus Bernadett,
Pató Zsuzsanna,
Sarang Zsolt,
Albert Réka,
Tőzsér József,
Petrovski Goran,
Csősz Éva
Publication year - 2017
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12252
Subject(s) - proliferative vitreoretinopathy , dispase , knockout mouse , tandem mass spectrometry , proteomics , retinal , downregulation and upregulation , retinal detachment , ophthalmology , medicine , chemistry , biochemistry , mass spectrometry , enzyme , chromatography , receptor , collagenase , gene
Proliferative vitreoretinopathy ( PVR ) develops as a complication of retinal detachment surgery and represents a devastating condition leading to serious vision loss. A good animal model that permits extensive functional studies and drug testing is crucial in finding better therapeutic modalities for PVR . A previously established mouse model, using dispase injection, was analyzed from the proteomic point of view, examining global protein profile changes by 2D electrophoresis, image analysis and HPLC –tandem mass spectrometry‐based protein identification. The easy applicability of the mouse model was used to study the role of transglutaminase 2 ( TG 2) in PVR formation by proteomic examination of dispase‐induced TG 2 knockout vitreous samples. Our data demonstrate that, despite the altered appearance of crystallin proteins, the lack of TG 2 did not prevent the development of PVR .