Open AccessExpression profiles of Br MYB transcription factors related to glucosinolate biosynthesis and stress response in eight subspecies of Brassica rapa
Author(s) -
Seo MiSuk,
Jin Mina,
Sohn SeongHan,
Kim Jung Sun
Publication year - 2017
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12231
Subject(s) - brassica rapa , biology , subspecies , pectobacterium carotovorum , polyploid , brassicaceae , glucosinolate , botany , biotic stress , gene , genome , genetics , abiotic stress , brassica , zoology
Brassica rapa is a polyploid species with phenotypically diverse cultivated subspecies. Glucosinolates ( GSL s) are secondary metabolites that contribute to anticarcinogenic activity and plant defense in Brassicaceae. Previously, complete coding sequences of 13 Br MYB transcription factors ( TF s) related to GSL biosynthesis were identified in the B. rapa genome. In the present study, we investigated GSL content and expression levels of these Br MYB TF s in 38 accessions belonging to eight subspecies of B. rapa . Twelve identified GSL s were detected and were classified into three chemical groups based on patterns of GSL content and expression profiles of the Br MYB TF s. GSL content and Br MYB TF expression levels differed among genotypes, including B. rapa subspecies pekinensis , chinensis and rapa . Br MYB 28.3 , Br MYB 51.1 and Br MYB 122.2 positively regulated GSL content in 38 accessions. Furthermore, expression levels of Br MYB 28 s and Br MYB 34.3 increased under most abiotic and biotic stress treatments. The three Br MYB 51 paralogs also showed drastically increased expression levels after infection with Pectobacterium carotovorum . The results of the present study improve our understanding of the functional diversity of these 13 Br MYB TF s during the evolution of polyploid B. rapa .