Open AccessSpontaneous formation of neutrophil extracellular traps in serum‐free culture conditions
Author(s) -
Kamoshida Go,
KikuchiUeda Takane,
Nishida Satoshi,
TanshoNagakawa Shigeru,
Kikuchi Hirotoshi,
Ubagai Tsuneyuki,
Ono Yasuo
Publication year - 2017
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12222
Subject(s) - neutrophil extracellular traps , innate immune system , extracellular , immune system , microbiology and biotechnology , bacteria , chemistry , mechanism (biology) , reactive oxygen species , biology , immunology , inflammation , genetics , philosophy , epistemology
Neutrophils play a critical role in the innate immune response. Recently, a new neutrophilic biological defense mechanism, termed neutrophil extracellular traps ( NET s), has been attracting attention. Neutrophils have been observed to release both lysosomal enzymes and their nuclear contents, including unfolded chromatin, which together trap and inactivate bacteria. The environment in tissues where neutrophils act is thought to be different from that of the blood serum. In this study, we assessed the effect of serum on NET formation. We found that neutrophils spontaneously form NET s in serum‐free cultivation conditions at early times. These NET s functioned properly to trap bacteria. Furthermore, we demonstrated that reactive oxygen species play a critical role in the spontaneous formation of NET s. These results suggest that the serum condition must be considered in studies on neutrophils, including the formation and mechanism of action of NET s.