Open AccessMiR‐29a‐3p suppresses cell proliferation in laryngocarcinoma by targeting prominin 1
Author(s) -
Su Jili,
Lu Eryong,
Lu Lijuan,
Zhang Chao
Publication year - 2017
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1002/2211-5463.12199
Subject(s) - prom , microrna , cell growth , transfection , biology , luciferase , cell , reporter gene , gene expression , microbiology and biotechnology , cancer research , cell culture , gene , medicine , genetics , obstetrics
MicroRNAs (miRNAs) are known to play a regulatory role in various cancers including laryngocarcinoma. MiR‐29a‐3p is a potential tumor‐suppressive mi RNA , but its function in laryngocarcinoma is unknown. The purpose of this study was to investigate the roles of miR‐29a‐3p in laryngocarcinoma. Prominin1 ( PROM 1) was predicted as a target gene of miR‐29a‐3p and this was verified using a luciferase reporter assay. Transfection of miR‐29a‐3p into two laryngocarcinoma cell lines indicated that miR‐29a‐3p could decrease cell proliferation and enhance the chemotherapy response by targeting PROM 1. PROM 1 expression was up‐regulated in the laryngocarcinoma cells when miR‐29a‐3p was down‐regulated. We found miR‐29a‐3p expression levels were lower in laryngocarcinoma tissues than in control tissues. We also found that miR‐29a‐3p expression was negatively correlated with PROM 1 expression in laryngocarcinoma tissues. The study demonstrates that miR‐29a‐3p suppresses cell proliferation in laryngocarcinoma by targeting PROM 1.